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Concentration
3-5u/μl 
5'…R↓GATCY…3' 
3'…YCTAG↑R…5'

Reaction Conditions 
1X Buffer V2
10mM Tris-HCl (pH 7.5 at 30°C), 10mM MgCl₂, 50mM NaCl, and 100μg/ml BSA. Incubate at 60°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 100mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol and 50% glycerol. Store at -20°C.

Thermal Inactivation
None.

Ligation / Recutting Assay 
After 5-fold overdigestion with BstX2I, more than 95% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 6u of BstX2I for 16 hours at 60°C.
Supplied with 10X Buffer V2, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

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Manual
BstX2I {XhoII}