Concentration
20-40u/μl
5'…GAT↓ATC…3'
3'…CTA↑TAG…5'
Reaction Conditions
1X Buffer EcoRV
10mM Tris-HCl (pH 8.5 at 30°C), 10mM MgCl2, 100mM NaCl, and 100μg/ml BSA. Incubate at 37°C.
Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 200μg/ml BSA and and 50% glycerol. Store at -20°C.
Thermal Inactivation
None.
Ligation / Recutting Assay
After 20-fold overdigestion with EcoRV, 80% of the DNA fragments can be ligated and recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of EcoRV for 16 hours at 37°C.
Supplied with 10X Buffer EcoRV, 10X Buffer UB and Viva Buffer A. (Diluent)
* High enzyme concentration may result in Star Activity.
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
RE1262
|
EcoRV
|
1000u
|
Downloads
Manual
EcoRV
