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Concentration
5-20u/μl 
5'…CA↓TATG…3'
3'…GTAT↑AC…5'

Reaction Conditions 
1X Buffer V5
30mM Tris-acetate (pH 7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 5-fold overdigestion with FauNDI, 60% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of FauNDI for 16 hours at 37°C. 
Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent)
* Sensitive to impurities present in some DNA preparation.

Ordering Information

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Manual
FauNDI {NdeI}