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Concentration
1-20u/μl
5'…GGC↓GCC…3'
3'…CCG↑CGG…5'

Reaction Conditions 
1X Buffer V4 
10mM Tris-HCl (pH 8.5 at 30°C), 10mM MgCl₂, 100mM KCl, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 250mM KCl, 0.1mM EDTA, 1mM DTT, 100μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 6-fold overdigestion with DinI, 70% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of DinI for 16 hours at 37°C. 
Supplied with 10X Buffer V4, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

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Manual
DinI {NorI}