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Concentration
5-10u/μl
5'…TCG↓CGA…3'
3'…AGC↑GCT…5'

Reaction Conditions 
1X Buffer V4
10mM Tris-HCl (pH 8.5 at 30°C), 10mM MgCl₂, 100mM KCl, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 250mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, and 50% glycerol. Store at -20°C.

Thermal Inactivation
None.

Ligation / Recutting Assay 
After 10-fold overdigestion with BtuM I, about 50% of the DNA fragments can be ligated and of these 90% can be recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 5u of BtuMI for 16 hours at 37°C. 
Supplied with 10X Buffer V4, 10X Buffer UB and Viva Buffer A. (Diluent) 
*Blocked by overlapping dam-methylation (G^mATC): GATCGCGA, (TCGCGATC).

Ordering Information

Downloads
Manual
BtuMI {NruI}