Concentration
5-20u/μl
5'…GTY↓RAC…3'
3'…CAR↑YTG…5'
Reaction Conditions
0.5X Buffer UB
12.5mM Tris-acetate (pH7.6 at 30°C), 5mM Mg-acetate, 50mM K-acetate, 3.5mM 2-mercaptoethanol and 25μg/ml BSA. Incubate at 37°C.
Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.
Thermal Inactivation
65°C for 20 minutes.
Ligation / Recutting Assay
After 10-fold overdigestion with HindIl, more than 60% of the DNA fragments can be ligated and recut. In the presence of 10% PEG, ligation is better.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 20u of HindII for 16 hours at 37°C
Supplied with 10X Buffer UB and Viva Buffer A. (Diluent)
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
RE1274
|
HindII
|
400u
|
Downloads
Manual
HindII
