Concentration
0.5-3u/μl
5'…GAAGA (N) 8↓…3'
3'…CTTCT (N) 7↑…5'
Reaction Conditions
1X Buffer MboII
33mM Tris-acetate (pH 7.9 at 30°C), 10mM Mg-acetate, 66mM K-acetate, and 1mM DTT. Incubate at 37°C.
Storage Buffer
10mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 100μg/ml BSA and 50% glycerol. Store at -20°C.
Thermal Inactivation
65°C for 20 minutes.
Ligation / Recutting Assay
After 3-fold overdigestion with MboII, about 60% of the DNA fragments can be ligated and recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 3u of MboII for 16 hours at 37°C.
Supplied with 10X Buffer MboII, 10X Buffer UB and Viva Buffer A. (Diluent)
*Blocked by overlapping dam-methylation (GmATC): GAAGATC
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
RE1290
|
MboII
|
100u
|
Downloads
Manual
MboII
