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Concentration
3-10u/μl 
5'…GAC↓GTC…3' 
3'…CTG↑CAG…5'

Reaction Conditions 
1X Buffer UB 
25mM Tris-acetate (pH 7.6 at 30°C), 10mM Mg-acetate, 100mM K-acetate, 7mM 2-mercaptoethanol and 50μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 10mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
80°C for 20 minutes.

Ligation / Recutting Assay 
After 5-fold overdigestion with ZraI, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 4u of ZraI for 16 hours at 37°C. 
Supplied with 10X Buffer UB and Viva Buffer A. (Diluent) 
* High enzyme concentration may result in Star Activity.

Ordering Information

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Manual
ZraI {AatII*}