最近最新的日本中文MV_麻豆出品视频在线_超pen个人视频97_国产精品第一国产精品_国产香蕉片_免费看麻豆文化传媒_奶大灬舒服灬太大了一进一出_人妻激情偷乱一区二区三区

Concentration
1-3u/μl
5'…C↓CCGGG…3' 
3'…GGGCC↑C…5'

Reaction Conditions 
1X Buffer V5 
30mM Tris-acetate (pH 7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 3-fold overdigestion with XmaI, more than 95% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 2u of XmaI for 16 hours at 37°C. 
Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

Downloads
Manual
XmaI {SmaI*}