Concentration
10-20u/μl
5'…GGTAC↓C…3'
3'…C↑CATGG…5'
Reaction Conditions
1X Buffer V1
10mM Tris-HCl (pH 7.5 at 30°C), 10mM MgCl2, and 100μg/ml BSA. Incubate at 37°C.
Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.
Thermal Inactivation
None.
Ligation / Recutting Assay
After 20-fold overdigestion with KpnI, more than 90% of the DNA fragments can be ligated and recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 20u of KpnI for 16 hours at 37°C.
Supplied with 10X Buffer V1, 10X Buffer UB and Viva Buffer A. (Diluent)
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
RV1286
|
KpnI
|
1000u
|
Downloads
Manual
KpnI
