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Concentration
1-10u/μl 
5'…G↓TGCAC…3'
3'…CACGT↑G…5'

Reaction Conditions 
1X Buffer V3
10mM Tris-HCl (pH 7.5 at 30°C), 10mM MgCl₂, 10mM NaCl, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes

Ligation / Recutting Assay 
After 10-fold overdigestion with VneI, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of Vnel for 16 hours at 37°C. 
Supplied with 10X Buffer V3, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

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Manual
VneI {ApaLI}