Concentration
4-20u/μl
5'…C↓TTAAG…3'
3'…GAATT↑C…5'
Reaction Conditions
1X Buffer V3
50mM Tris-HCl (pH7.5 at 30°C), 10mM MgCl2, 100mM NaCl, and 100μg/ml BSA. Incubate at 37°C.
Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.
Thermal Inactivation
65°C for 20 minutes.
Ligation / Recutting Assay
After 5-fold overdigestion with Vha464I, 40% of the DNA fragments can be ligated and recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 4u of Vha464I for 16 hours at 37°C.
Supplied with 10X Buffer V3, 10X Buffer UB and Viva Buffer A. (Diluent)
Ordering Information
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Catalog No
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Description
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Pack Size
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|
RE1358
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Vha464I {AftII}
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600u
|
Downloads
Manual
Vha464I {AftII}
Publication
This Product Has Been Used In:
Khademi, K.G., et al. (2016) Association Study of rs1333040 and rs1004638 Polymorphisms in the 9p21 Locus with Coronary Artery Disease in Southwest of Iran. Iranian Biomedical Journal. 20(2), p.122-127.
