Concentration
10-20u/μl
5'…T↓TAA…3'
3'…AAT↑T…5'
Reaction Conditions
1X Buffer V1
10mM Tris-HCl (pH 7.5 at 30°C), 10mM MgCl2, and 100μg/ml BSA. Incubate at 65°C.
Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 1mM DTT, 200μg/ml BSA and 50% glycerol. Store at -20°C.
Thermal Inactivation
None.
Ligation / Recutting Assay
After 10-fold overdigestion with Tru9 I, more than 95% of the DNA fragments can be ligated and recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 20u of Tru9I for 16 hours at 65°C.
Supplied with 10X Buffer V1, 10X Buffer UB and Viva Buffer A. (Diluent)
*Blocked by TTAmA methylation
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
RE1350
|
Tru9I
|
200u
|
Downloads
Manual
Tru9I
