Concentration
2-10u/μl
5'…↓AATT…3'
3'…TTAA↑…5'
Reaction Conditions
1x Buffer V1
10mM Tris-HCl (pH7.5 at 30°C), 10mM MgCl2, and 100μg/ml BSA. Incubate at 55°C.
Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 100μg/ml BSA and 50% glycerol. Store at -20°C.
Thermal Inactivation
65°C for 20 minutes.
Ligation / Recutting Assay
After 5-fold overdigestion with Sse9I, more than 95% of the DNA fragments can be ligated and recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of Sse9I for 16 hours at 55°C.
Supplied with 10X Buffer V1, 10X Buffer UB and Viva Buffer A. (Diluent)
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
RE1342
|
Sse9I
|
200u
|
Downloads
Manual
Sse9I
