Concentration
5-20u/μl
5'…AAT↓ATT…3'
3'…TTA↑AAT…5'
Reaction Conditions
1X Buffer SspI
10mM Tris-HCl (pH 7.6 at 30°C), 10mM MgCl2, 100mM KCl, and 100μg/ml BSA. Incubate at 37°C.
Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, and 50% glycerol. Store at -20°C.
Thermal Inactivation
65°C for 20 minutes.
Ligation / Recutting Assay
After 10-fold overdigestion with SspI, 90% of the DNA fragments can be ligated and recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 5u of SspI for 16 hours at 37°C.
Supplied with 10X Buffer Ssp I, 10X Buffer UB and Viva Buffer A. (Diluent)
*High enzyme concentration may result in Star Activity.
*Blocked by AmATATT methylation
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
RE1344
|
SspI
|
200u
|
Downloads
Manual
SspI
Publication
This Product Has Been Used In:
Rafiei, A., et al. (2014) Molecular Characterization of Crytosporidium spp. Isolated from Immunocomprmised Patients and Children. Jundishapur Journal of Microbiology. 7(4).
