Concentration
5-10u/μl
5'…CAYNN↓NNRTG…3'
3'…GTRNN↑NNYAC…5'
Reaction Conditions
1X Buffer V4
10mM Tris-HCl (pH 8.5 at 30°C), 10mM MgCl2, 100mM KCl, and 100μg/ml BSA. Incubate at 37°C.
Storage Buffer
10mM Tris-HCl (pH 7.5), 250mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.
Thermal Inactivation
65°C for 20 minutes.
Ligation / Recutting Assay
After 10-fold overdigestion with SmiMI, 90% of the DNA fragments can be ligated and recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of SmiMI for 16 hours at 37°C.
Supplied with 10X Buffer V4, 10X Buffer UB and Viva Buffer A. (Diluent)
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
RE1378
|
SmiMI
|
100u
|
Downloads
Manual
SmiMI
