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Concentration
10-30u/μl
5'…ATTT↓AAAT…3'
3'…TAAA↑TTTA…5'

Reaction Conditions 
1X Buffer V3
50mM Tris-HCl (pH 7.5 at 30°C), 10mM MgCl₂, 100mM NaCl, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 250mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 100μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 10-fold overdigestion with SmiI, more than 95% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of SmiI for 16 hours at 37°C. 
Supplied with 10X Buffer V3, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

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Manual
SmiI