Concentration
5-15u/μl
5'…GCATC(N)5↓…3'
3'…CGTAG(N)9↑…5'
Reaction Conditions
1X Buffer V3
50mM Tris-HCl (pH 7.5 at 30°C), 10mM MgCl2, 100mM NaCl, and 100μg/ml BSA. Incubate at 37°C.
Storage Buffer
10mM Tris-HCl (pH 7.5), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 200μg/ml BSA and 50% glycerol. Store at -20°C.
Thermal Inactivation
None.
Ligation / Recutting Assay
After 10-fold overdigestion with SfaNI, more than 90% of the DNA fragments can be ligated and recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 15u of SfaNI for 16 hours at 37°C.
Supplied with 10X Buffer V3, 10X Buffer UB and Viva Buffer A. (Diluent)
*Blocked by CpG methylation
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
RE1376
|
SfaNI
|
200u
|
Downloads
Manual
SfaNI
