Concentration
2-5u/μl
5'…CCTGCA↓GG…3'
3'…GG↑ACGTCC…5'
Reaction Conditions
1X Buffer V5
30mM Tris-HCl (pH 7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg/ml BSA. Incubate at 37°C.
Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.
Thermal Inactivation
80°C for 20 minutes.
Ligation / Recutting Assay
After 5-fold overdigestion with SbfI, more than 90% of the DNA fragments can be ligated and of these 90% can be recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 5u of SbfI for 16 hours at 37°C.
Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent)
* High enzyme concentration may result in Star Activity.
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
RE1328
|
SbfI
|
100u
|
Downloads
Manual
SbfI
