Concentration
5-10u/μl
5'…GCATG ↓C…3'
3'…C↑GTACG…5'
Reaction Conditions
1X Buffer UB
25mM Tris-acetate (pH 7.6 at 30°C), 10mM Mg-acetate, 100mM K-acetate, 7 mM 2-mercaptoethanol, and 50μg/ml BSA. Incubate at 37°C.
Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.
Thermal Inactivation
65°C for 20 minutes.
Ligation / Recutting Assay
After 3-fold overdigestion with Sphl, 90% of the DNA fragments can be ligated and recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 6u of Sphl for 16 hours at 37°C.
Supplied with 10X Buffer UB and Viva Buffer A. (Diluent)
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
RV1340
|
Sphl
|
200u
|
Downloads
Manual
Sphl
