Concentration
20-100u/μl
5'…CTGCA↓G…3'
3'…G↑ACGTC…5'
Reaction Conditions
1X Buffer V3
50mM Tris-HCl (pH 7.5 at 30°C), 10mM MgCl2, 100mM NaCl, and 100μg/ml BSA. Incubate at 37°C.
Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, and 50% glycerol. Store at -20°C.
Thermal Inactivation
None.
Ligation / Recutting Assay
After 30-fold overdigestion with PstI, more than 90% of the DNA fragments can be ligated and recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of PstI for 16 hours at 37°C.
Supplied with 10X Buffer V3, 10X Buffer UB and Viva Buffer A. (Diluent)
* High enzyme concentration may result in Star Activity.
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
RE1320
|
PstI
|
2000u
|
Downloads
Manual
PstI
