Description
2X Taq Master Mix is an optimized ready-to-use 2X concentrated DNA amplification mixture containing Taq DNA polymerase, reaction buffer, dNTPs and MgCl2. It contains all the components required for routine DNA amplification, except template and primers.
Features
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Saves time and reduces contamination due to reduced number of pipetting steps.
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Stable at 4°C for 6 months, allowing immediate reaction setup without the time consuming thawing of reagents.
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Suitable for all routine DNA amplification applications.
Composition
Taq DNA Polymerase (0.05u/μl), 2X ViBuffer A (100mM KCl, 20mM TrisHCl (pH9.1 at 20°C) and (0.02% Triton? X-100), 0.4mM dNTPs and 3.0mM MgCl2.
Supplied With
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50mM MgCl2
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Nuclease-free Water
Quality Control
All preparations are assayed for contaminating endonuclease, exonuclease, and non-specific DNase activities. Functionally tested in DNA amplification.
Storage & Stability
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Stable at -20°C for one year or at 4°C for 6 months if properly stored.
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Stable for 20 freeze-thaw cycles. To avoid frequent freeze-thaw, keeping small aliquots at -20°C is recommended.
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For daily use, keeping aliquots at 4°C is recommended.
Ordering Information
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Catalog No
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Description
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Pack Size
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PLMM01
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2X Taq Master Mix
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100 applications
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Download
Manual
2X Taq Master Mix
Comparison Test Report
2X Taq Master Mix
Publication
This Product Has Been Used In:
Boonyayatra, S., Tharavichitkut, P., Oliver, S.P. (2018). Virulence-associated genes and molecular typing of Streptococcus uberis associated with bovine mastitis in northern Thailand, Turkish Journal of Veterinary and Animal Sciences, Vol. 42, 73-81 (2018)
Azhar, N.S., Zin, N.H.M., Abdul-Hamid, T.H.T. (2017). Lactococcus Lactis Strain A5 Producing Nisin-like Bacteriocin Active against Gram Positive and Negative Bacteria, Tropical Life Sciences Research, Vol. 28, No. 2 (2017).
Ajijolakewu, K.A., Leh, C.P., Wan-Abdullah, W.N., Lee, C.K. (2016). Assessment of the Effect of Easily-metabolised Carbon Supplements on Xylanase Production by Newly Isolated Trichoderma asperellum USM SD4 Cultivated on Oil Palm Empty Fruit Bunches. BioResources,Vol. 11, No. 4, 9611-9627 (2016).
Mohamad, Y., Reda, W.W., Abdel-Moein, K., El-Razil, K.A.A., Barakat, A.M.A., El Fadaly, H.A., Hassanain, N.A., Hegazi, A.G. (2016) Prevalence and phylogenetic characterization of Listeria Monocytogenes isolated from processed meat marketed in Egypt.. Journal of Genetic Engineering and Biotechnology. 14(1). Pp.119-123
Allahverdi, A., et al. (2015) Differentiation of Human Mesenchymal Stem Cells into Insulin Producing Cells by Using A Lentiviral Vector Carrying PDX1. CELL Journal.17(2), p.231-242.
Feh, C.F., Wang, K.C., Lu, C.Y., Chiang, L.C., Shieh, D.E., Yen, M.H., Chang, J.S. (2015) Yakammaoto inhibits enterovirus 71 infection by reducing viral attachment, internalization, replication, and translation. Kaohsiung Journal of Medical Sciences. 31. Pp.293-302.
Sabourmoghaddam, N. Zakaria, M.P., Omar, D. (2015) Evidence for the microbial degradation of imidacloprid in soils of Cameron Highlands. Journal of Saudi Society of Agricultural Sciences.14(2) pp.182-188.
Sabourmoghaddam, N. et al.(2015) Evidence for Microbial Degradation of Imidacloprid in Soils of Cameron Highlands. Journal of Saudi Society of Agricultural Sciences. ScienceDirect. 14(2), p. 182-188.
Wiparat,S., Poeaim, S., Eiamampai, K., Atittayawan (2015). Gender Identification of Himantopus Himantopus Using PCR-Based Method, International Journal of Agricultural Technology, Chiang Mai Journal of Science, Vol. 11, No.2, 307-314 (2015).
Belgini, D.R.B., et al (2014) Culturable Bacterial Diversity from a Feed Water of a Reverse Osmosis System, Evaluation of Biofilm Formation and Biocontrol Using Phages. World Journal of Microbiology and Biotechnology. ProQuest. 30, p. 2689-2700.
Belkahia, H., Said, M.B., Hamdi, S.E., Yahiaoui, M., Gharbi, M., Daaloul-Jedidi, M., Mhadbi, M., Jedidi, M., Darghout, M.A., Klabi, I., Zribi, L., Messadi, L. (2014) ) First molecular identification and genetic characterization ofAnaplasma ovis in sheep from Tunisia. Small Ruminant Research. 121. Pp.404-410.
Dhiritiman, C., Sharma, G.D., Jha, D.K., Hijri, M. (2014). Associations of Arbuscular Mycorrhizal (AM) fungi in the Phytoremediation of Trace Metal (TM) Contaminated Soils, Journal of Research in Biology, Vol. 4, No. 2 (2014).
Reyes, J.C.B., Solon, J.A.A., Rivera, W.L. (2014) The analysis of correlation between IL-1B gene expression and genotyping in multiple sclerosis patients. Microbiology and Infectious Disease 179. Pp..337-341.
Hipol, R.M. (2012) Molecular Identification and Phylogenetic Affinity of Two Growth Promoting Fungal Endophytes of Sweet Potato (Ipomea batatas (L.) Lam.) from Baguio City, Philippines. Electric Journal of Biology, 8(3): 57-61.
