Description
Flex-C Cloning Kit is a highly efficient, rapid, and easy-to-use PCR cloning kit. The Flex-C Enzyme allows direct cloning of any PCR fragments into any linearized expression vector at any site in a single 20-minute reaction.
The application protocol is simple. The PCR fragments can be generated by PCR Polymerases (for eg. Taq DNA Polymerase) with primers that are designed to have at least 10 bases of homology with the vector at their linear ends. No additional treatment of the PCR fragment is required (such as restriction digestion, ligation, phosphorylation, or blunt-end polishing). The linearized vector can be generated by PCR or restriction enzymes (single or double digestion). Flex-C Enzyme joins PCR fragments and linearized vectors accurately and efficiently by recognizing the 10bp overlap at their ends.
This method allows cloning of multiple fragments into a single vector in a single reaction, without subcloning, to create fusion proteins, to delete and replace DNA sequences or to insert point mutations. The Flex-C Cloning Kit is highly efficient with a 95% insert rate. The Flex-C Cloning Kit also includes Vivantis Taq DNA Polymerase and reaction buffers as well as dNTPs for subsequent PCR screening of clones.
Features
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Clone any insert, at any site within any vector
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Restriction enzyme, phosphatase and ligase-free system
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Joining multiple fragments at once
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Broad PCR size up to 10kb
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Good for 5’ overhangs, 3’ overhangs, blunt ends
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Precise insertion at a desired orientation
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High efficiency with > 95% positive clones
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Multiple applications:
-Adding adaptor, linker and tag before or after the insert
-Mutation generation
-Gene synthesis
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High throughput application
Kit Components
Flex-C Cloning Kit (Without Competent Cell)
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Flex-C Cloning Enzyme
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Taq DNA Polymerase and buffers
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10mM dNTP Mix
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Nuclease-free water
Flex-C Cloning Kit (With Competent Cell)
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Flex-C Cloning Enzyme
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Competent Cells (1X109cfu/μg)
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Taq DNA Polymerase and buffers
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10mM dNTP Mix
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Nuclease-free water
Storage & Stability
Store at -20°C.
Ordering Information
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Catalog No
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Description
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Pack Size
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MEPL01
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Flex-C Cloning Kit (Without Competent Cell)
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20 applications
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MEPL02
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Flex-C Cloning Kit (With Competent Cell)
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20 applications
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Download
Manual
Flex-C Cloning Kit (Without Competent Cell)
Flex-C Cloning Kit (With Competent Cell)
