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Description
ViPrimePLUS One Step AtTaq RT-qPCR Green Master Mix I is next generation first choice mix designed for fast and easy one step real-time PCR reaction set up. The improved formulation of master mix contains unique thermostable MMULV enzyme, Hot Start Taq DNA Polymerases, SYBR® Green dye as well as MgCl2 and buffer components at optimal concentrations. The M-MULV enzyme has an optimal operating temperature and higher affinity for primer template duplexes which allows very rapid processing during RT step. SYBR® Green dye emits fluorescence when bound to double-stranded DNA. Detection of PCR product is monitored by the increase in fluorescence, leading to high sensitivity, wide dynamic range and high reproducibility for quantification.
ViPrimePLUS One Step AtTaq RT-qPCR Green Master Mix I can be used to amplify any RNA template including mRNA, total RNA and viral sequences. The improved formulation of RT-qPCR master mix can detect extremely low copy number targets very specifically with high efficiency that give CT values close to the theoretical time of detection. The ViPrimePLUS One Step AtTaq RT-qPCR Green Master Mix I is a complete system for use in one step realtime PCR, the removal of a separate reverse transcription step reduces handling errors as well as the time taken to obtain results. The formation of primer dimers and nonspecific products is prevented leading to optimum sensitivity and specificity.
ViPrimePLUS One Step AtTaq qRT-PCR Green Master Mix I has several formulations optimized to be used with most of real-time PCR instruments. The improved sensitivity and consistency of ViPrimePLUS One Step AtTaq RT-qPCR Green Master Mix I in standard cycling conditions gives the industry leading performance in fast cycling conditions.

Applications
All kinds of RNA sample material suited for RT-qPCR amplification can be used.

Features

• One step real time RT-qPCR reaction set up
• Equipped with thermostable M-MULV enzyme and Hot Start Taq DNA Polymerases
• Includes SYBR® Green dye for intercalator-based qPCR
• Reliable quantification of extremely low copy number targets
• Optimal performance for highly sensitive and specific RT-qPCR reaction
• Compatible with most of the real-time PCR platforms

Component
3 x 0.6ml aliquots of master mix 0.6ml aliquots of “no RT control master mix standard”

Storage
Stable at -20°C up to the expiry date stated. Store all components at -20°C upon arrival. Keep in aliquot to reduce freeze-thaw cycles.

Quality Control
As part of the ISO9001:2008 quality assurance systems, each lot of ViPrimePLUS One Step AtTaq RT-qPCR Green Master Mix I has been tested against predetermined specifications to ensure consistent product quality and highest levels of performance and reliability.

Limitation Of Use
For research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals.

Instruments
To calibrate a real-time PCR reaction, various formulations of master mixes are available for most of the platforms.

Ordering Information

Download
Manual

ViPrimePLUS One Step AtTaq RT-qPCR Green MasterMix I with ROX (SYBR® Green Dye) 
ViPrimePLUS One Step AtTaq RT-qPCR Green MasterMix I with Low ROX (SYBR® Green Dye) 
ViPrimePLUS One Step AtTaq RT-qPCR Green MasterMix I with ROX (SYBR® Green Dye)

Publication
This Product Has Been Used In: 
El Serafy, M.A., Sabry, D., El din, M. A. M., Moustafa, A., El Kazaz, A., Mohammed, S., R., Abou-Elalla, A.A. (2017). Association of SIRT-1, T helper 17-associated gene and Antimicrobial Peptides Genes in Inflammatory Bowel Disease Patients. . . International Journal of Biomedicine. Vol. 7, No. 3 (2017) 196-203