Description
The ViPrimePLUS Epizootic Hemorrhagic Disease Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Epizootic Hemorrhagic Disease Virus genome in tissue samples such as spleen, lymph node, liver and lungs, and unclotted whole blood.
Epizootic Hemorrhagic Disease Virus (EHDV) is a single stranded RNA virus which is responsible for the epizootic hemorrhagic disease a.k.a. Ibaraki disease in deer, especially the white-tailed deer. EHD occurs in North American, Australia, Asia and Africa. There are 10 serogroups identified where each has different geographical distribution. Some of them can also affect cattle and most of other ruminants. The Ibaraki virus is the EHD serotype 2 discovered from the Japan, Korea and Taiwan outbreaks. The virus is very contagious and spreads very quickly in a herd of deer. There are 3 forms of syndromes observed in the infected deer; 1) Peracute form where high fever, anorexia, respiratory distress and edema of the head are seen, 2) Acute disease which is accompanied by haemorrhages in the skin, heart and gastrointestinal tract, and the 3) Chronic form where the recovered deer develop abnormal hooves, which paralyzes them, and ulceration in the rumen. No vaccines are available for EHDV, except for Ibaraki virus which is currently being used in Japan.
ViPrimePLUS Epizootic Hemorrhagic Disease Virus RT-qPCR Kit is intended for research use only.
Kit Component
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EHDV Primers and Probe Mix (EHDV PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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RNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Ruminant species samples (e.g. spleen, lymph node, liver and lungs, unclotted whole blood)
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Gene Target
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NS1 gene
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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? Internal Extraction Control (IEC) as inhibition control
? Positive control
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Channels
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? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QV7002
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ViPrimePLUS Epizootic Hemorrhagic Disease Virus RT-qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Epizootic Hemorrhagic Disease Virus RT-qPCR Kit
