Description
ViPrimePLUS Lyophilized One Step qRT-PCR Master Mix is next generation first choice mix for a convenient and rapid one step real-time PCR reaction set up. The master mix has been freeze-dried to produce a room temperature stable preparation. The freeze-dried master mix contains unique thermostable M-MULV enzyme, Taq DNA polymerases, MgCl2 and buffer components at optimal concentration.
ViPrimePLUS Lyophilized One Step qRT-PCR Master Mix has same optimal performance as ViPrimePLUS One Step qRT-PCR Master Mix and ViPrimePLUS One Step qRT-PCR Green Master Mix. The lyophilized qRT-PCR Master Mix can detect extremely low copy number targets of RNA templates in one step complete system. The removal of separate reverse transcription step not only reduces errors and time to obtain results, but also leads the qRT-PCR reaction to optimum sensitivity and specificity.
ViPrimePLUS Lyophilized One Step qRT-PCR Master Mix is used with supplied re-suspension buffer and ROX dye; hence hardware platforms that need ROX as passive reference dye can be used in standard cycling conditions.
Features
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Convenient and rapid real time PCR reaction set up
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Lyophilized form – room temperature stable preparation
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Equipped with thermostable M-MULV enzyme and Taq DNA Polymerase
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Reliable quantification of low-abundance transcripts very specifically with high efficiency
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Compatible with most of the real-time PCR platforms
Kit Component
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3 x Lyophilized qPCR MasterMix
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1 x Lyophilized ROX
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4 x Re-suspension buffer
Master Mixes Selection Guidelines for System Compatibility
To calibrate a real-time PCR reaction, the various formulations of Master Mixes are available for most of the platforms.
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Real-time PCR platforms
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ROX re-suspension volume
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ROX addition per tube of master mix
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Applied Biosystems 7000, 7700, 7900, 7300 StepOne, StepOnePLUS and ViA7 platforms, Roche capillary Lightcyclers.
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100μl
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20μl
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All Stratagene platforms
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200μl
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15μl
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Applied Biosystems 7500 platform
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700μl
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10μl
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All other machines
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Not Required
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Not Required
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Ordering Information
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Catalog No
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Description
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Pack Size
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QLMM06
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ViPrimePLUS Lyophilized One Step qRT-PCR Master Mix
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150 reactions
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Download
Manual
ViPrimePLUS Lyophilized One Step qRT-PCR Master Mix
