Description
The ViPrimePLUS African Horse Sickness Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of African Horse Sickness virus genomes in blood samples.
African Horse Sickness is an infectious, noncontagious disease that affects horses, mules, zebras and donkeys. Mules and horses in particular are most vulnerable to this disease. The responsible pathogen, the African Horse Sickness virus (AHSV) is a double-stranded RNA virus with a family of nine serotypes, of which only the 9th serotype is endemic and widespread. The disease exhibits 4 distinct forms; 1) pulmonary form which causes serious lung congestion that leads to lung failure and death, 2) cardiac form which is characterized by edema around the face and neck with 50% mortality risk, 3) mixed form which exhibits a combination of both pulmonary and cardiac symptoms, resulting in 70-80% mortality risk, and 4) mild fever form which is rarely fatal and usually seen in donkeys and zebras. Thus, this is where real time PCR plays a role to provide a sensitive, non-serological test for disease screening.
ViPrimePLUS African Horse Sickness Virus RT-qPCR Kit is intended for research use only.
Kit Component
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AHSV Primers and Probe Mix (AHSV PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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DNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Horse, mule, zebra and donkey samples (e.g. blood)
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Gene Target
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VP7 gene
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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? Internal Extraction Control (IEC) as inhibition control
? Positive control
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Channels
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? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QV3001
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ViPrimePLUS African Horse Sickness Virus RT-qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS African Horse Sickness Virus RT-qPCR Kit
