Description
The ViPrimePLUS Equine Infectious Anaemia Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection Equine Infectious Anaemia Virus genome in peripheral blood and serum samples.
Equine infectious anaemia virus (EIAV) is a RNA virus that causes Swamp Fever in the members of the equid family, including horses, mules and donkeys. Horizontal transmission of the infection is via blood sucking flies and mosquitoes, while vertical transmission is possible, too as the virus can cross the placental barrier. Symptoms of the acute infection are recurrent fever episodes, weight loss, depression, anemia and edema. In the chronic stage, the infection tends to become inapparent and finally leads to a prolonged asymptomatic period, where the infected horses remain as viremic carriers for life. Thus, real time PCR technique can be a suitable to screen for these animals. Currently, vaccination is not available in the market.
ViPrimePLUS Equine Infectious Anaemia Virus RT-qPCR Kit is intended for research use only.
Kit Component
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EIAV Primers and Probe Mix (EIAV PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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DNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Horse, mule, and donkey (e.g. blood and serum)
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Gene Target
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Equi merozoite antigen 1 (ema-1) gene.
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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? Internal Extraction Control (IEC) as inhibition control
? Positive control
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Channels
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? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QV3005
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ViPrimePLUS Equine Infectious Anaemia Virus RT-qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Equine Infectious Anaemia Virus RT-qPCR Kit
