Description
The ViPrimePLUS Theileria equi qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Theileria equi genome in horse blood samples.
Equine piroplasmosis is tick-borne protozoal infection occurring in horses, mules, donkeys and zebras. Theileria equi (formerly known as Babesia equi) and Babesia caballi are the responsible pathogens. Although, the disease is endemic in tropical and subtropical regions, it has been reported in other countries and continents as well. Therefore, piroplasmosis is a major constraint to the international movement of equines. Among the disease symptoms are acute fever, inappetence, malaise, anemia, labored breathing, jaundice, sudden death, chronic weight loss and poor exercise tolerance. T. equi tends to cause more serious disease than B. caballi. Mortality rate was reported to be in a range from 10% to 50%. Although the tick vector is the primary mode of transmission, animals can be infected by contaminated syringes or needles and blood transfusion. Vertical transmission for T. equi to foals is possible, too. Recovered equids are potential long term carrier of the organisms. Other infected animals may be able to clear the organism eventually after several years. In the aspects of public health, B. caballi or T. equi may have been implicated in a few human infections in the past but these organisms do not seem to be important zoonoses. Babesia divergens and Babesia microti are the more common cause of human babesiosis.
ViPrimePLUS Theileria equi qPCR Kit is intended for research use only.
Kit Component
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T. equi Primers and Probe Mix (T. equi PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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DNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Horse, mule, donkey and zebra samples (e.g. blood)
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Gene Target
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Equi merozoite antigen 1 (ema-1) gene.
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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? Internal Extraction Control (IEC) as inhibition control
? Positive control
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Channels
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? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QV3006
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ViPrimePLUS Theileria equi qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Theileria equi qPCR Kit
