Description
The ViPrimePLUS Trypanosoma equiperdum qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Trypanosoma equiperdum genome in urine, urogenital and vaginal swab.
Trypanosoma equiperdum is a protozoan responsible for dourine disease among horses (most susceptible), mules and donkeys. Dourine is the only trypanosomiasis that is not spread via an invertebrate vector, but rather it is transmitted sexually, during coitus. The disease manifests various symptoms. Classically, it is characterized into three stages. The first stage will observed swelling of genitalia, abnormal vaginal discharges, patch discoloration in the vulva and penis. Fever and loss appetite may occur. The second stage follows after 4weeks, where intermittent onset of round urticarial formation appear around the neck, chest and flanks. In the third stage, paralysis of the limbs takes place, thus leading to death though the disease may persist for a year or two. However, most of the infected animals may only developed mild symptoms, especially those from endemic regions. Diagnosis is difficult as the trypanosomes are not present in blood, but in the genital tissue or fluids. Serological test could not differentiate between T. equiperdum and other trypanosomes, such as T. evansi and T. brucei. Thus, molecular diagnosis is a good alternative in terms of sensitivity and specificity.
ViPrimePLUS Trypanosoma equiperdum qPCR Kit is intended for research use only.
Kit Component
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A. duodenale Primers and Probe Mix (A. duodenale PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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DNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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?Horse, mule and donkey samples (e.g. urine, urogenital, vaginal swab)
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Gene Target
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Germline variant surface antigen (VSG-78 BC-3) pseudogene
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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? Internal Extraction Control (IEC) as inhibition control
? Positive control
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Channels
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? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QV3007
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ViPrimePLUS Trypanosoma equiperdum qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Trypanosoma equiperdum qPCR Kit
