Description
The ViPrimePLUS Fungi qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Fungi genomes in food, human, animal, plant and environmental samples.
Fungi refer to a broad range of organisms, consisting of yeast, molds and mushroom. They are eukaryotic organisms with rigid cell walls. The organism is ubiquitous in every niche in the environment and human are constantly exposed to them. Some of the pathogenic fungi produce bioactive compounds known as mycotoxins such as alkaloids and polyketides that are toxic to animals including humans. They bring about major economic losses due to the destruction of crops, food spoilage and various diseases in animals and humans. However, some fungi are beneficial to humans as they can be used directly as food source or contribute in the production of food, beverages, detergent, pesticides and antibiotics industries.
ViPrimePLUS Fungi qPCR Kit is intended for research use only.
Kit Component
-
Fungi Primers and Probe Mix (Fungi PPM)
-
Positive Control
-
Internal Extraction Control Primers and Probe Mix (IEC PPM)
-
Internal Extraction Control (IEC DNA)
-
Nuclease Free Water
-
Template Preparation Buffer
Product Specification
|
Technology
|
Taqman probe-based real-time PCR assay
|
|
Type of Nucleic Acid Kit
|
DNA
|
|
Kit Storage
|
Shipped in room temperature, store at -20℃ on arrival
|
|
Sample Material
|
? Human samples
? Animal samples
? Plant samples
? Food samples
? Environmental samples
|
|
Gene Target
|
18S ribosomal RNA gene
|
|
Detection Limit
|
10 to <100 copies per reaction
|
|
Sensitivity & Specificity
|
Ct value 28±3 with probability of 95%
|
|
Controls Included
|
? Internal Extraction Control (IEC) as inhibition control
? Positive control
|
|
Channels
|
? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
|
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
QM3008
|
ViPrimePLUS Fungi qPCR Kit
|
150 reactions
|
Download
Manual
ViPrimePLUS Fungi qPCR Kit
