Description
The ViPrimePLUS Encephalitozoon qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Encephalitozoon genomes in faeces, urine sediment or tissue samples.
Encephalitozoon is a single cell protozoan which commonly causes diseases in animals and humans. There are 4 species which infects humans, resulting in clinical symptoms such as E. intestinalis that causes diarrhea and cholecystits, E. cuniculi that causes encephalitis and E. hellum that causes keratoconjunctivitis. On the other hand, the zoonotic pathogen, E. cuniculi is also an important pathogen in rabbits, rodents, guinea pigs, foxes, cats and dogs. Three strains of E. cuniculi have been characterized, which are; type 1 from dogs, type 2 from rodents, type 3 from rabbits. Co-habitation with pets resulted in common infection from type 1 and type 3 in humans. Clinical manifestations are usually only found in immunocompromised individuals such as patients with HIV, organ transplant recipient and cancer patient undergoing chemotherapy. Approximately 8-10% of the AIDS population in the United States is clinically infected with E. intestinalis or Enterocytozoon bieneusi.
ViPrimePLUS Encephalitozoon qPCR Kit is intended for research use only.
Kit Component
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Encephalitozoon Primers and Probe Mix (Encephalitozoon PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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DNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Clinical samples (e.g. faeces, urine)
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Gene Target
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16S ribosomal RNA gene
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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? Internal Extraction Control (IEC) as inhibition control
? Positive control
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Channels
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? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QM3006
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ViPrimePLUS Encephalitozoon qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Encephalitozoon qPCR Kit
