Description
The ViPrimePLUS Aspergillus qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of all pathogenic subtypes of Aspergillus genomes in bronchoalveolar lavage fluids and blood or serum samples.
Aspergillus is a ubiquitous airborne fungus found in the indoor and outdoor environment. As a weak opportunistic pathogen, most human and animals who constantly inhale the aspergillus conidia showed no symptoms of illness as the human immune system quickly eliminates them. However, people with lung diseases and weakened immunity are more prone to developing allergic bronchopulmonary aspergillosis, aspergilloma and fatal invasive aspergillosis. Due to the rising number of immunocompromised patients, a 4-fold increase in the invasive aspergillosis (IA) has been observed in the last 12 years. A. fumigatus is the most common etiologic agent, being responsible for approximately 90% of human infections. Other agents include A. flavus, A. terreus, A. niger, and A. nidulanswhich can also cause human infections.
ViPrimePLUS Aspergillus qPCR Kit is intended for research use only.
Kit Component
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Aspergillus Primers and Probe Mix (Aspergillus PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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DNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Clinical samples (e.g. bronchoalveolar lavage fluids, blood, serum)
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Gene Target
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18S ribosomal RNA gene, transcribed spacer 1, 5.8S ribosomal RNA gene
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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? Internal Extraction Control (IEC) as inhibition control
? Positive control
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Channels
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? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QM3002
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ViPrimePLUS Botrytis cinerea qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Aspergillus qPCR Kit
