Description
The ViPrimePLUS Spring Viremia of Carp Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Spring Viremia of Carp Virus genome in fish samples (e.g. liver, kidney, gills, spleen and brain).
Spring Viremia of Carp Virus (SVCV), a bullet-shaped RNA virus, is responsible for the acute haemorrhagic and contagious viremia in several carp species, and some other cyprinid and ictalurid fish species. The virus has first been identified in Yugoslavia and now, most of the European countries, Russia, the Middle East and even China have been reported of the disease. The virus can be transmitted via the water contaminated with the faeces and urine of the infected carp or via invertebrate vectors. Once the virus enters the host via the gill, the virus spreads rapidly to the liver, kidney, spleen and digestive tract. Diseased fish is usually darker in colour with typical signs of exophthalmia, pale gills, haemorrhages on skin and base of fins, abdominal distension and a protruding anus. Disease usually manifests in water with temperature between 11 and 17oC. Therefore in carp, the disease is often observed during spring period. In European aquaculture, the mortality rate can reach up to 70% in yearling. Vaccine is currently not available, thus effective screening method is essential.
ViPrimePLUS Spring Viremia of Carp Virus RT-qPCR Kit is intended for research use only.
Kit Component
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SVCV Primers and Probe Mix (SVCV PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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RNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Fish samples (e.g. liver, kidney, gills, spleen, brain)
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Gene Target
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L2 inner capsid protein VP2
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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? Internal Extraction Control (IEC) as inhibition control
? Positive control
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Channels
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? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QV4005
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ViPrimePLUS Spring Viremia of Carp Virus RT-qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Spring Viremia of Carp Virus RT-qPCR Kit
