Description
The ViPrimePLUS Porcine Circovirus 2 qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Porcine Circovirus 2 genome in blood and tissues.
Porcine Circovirus (PCV) is a very small circular DNA virus that widely infects pigs. They are classified into PCV1 and PCV2, which differ in the ORF2 region that encodes for Cap. PCV1 was first discovered as a contaminant in a pig kidney cell line and it is a commonly present swine virus which has no known disease caused. Infection with PCV2 may cause typical PCVD lesions. Some PCV2 infected pigs may also develop the PCV-associated diseases (PCVAD) such as severe systemic PCV2 infection and Post-weaning Multisystemic Wasting Syndrome (PMWS), PCV2-associated pneumonia as part of the Porcine Respiratory Disease Complex (PRDC), PCV2-associated enteritis, PCV2-associated reproductive failure, and Porcine Dermatitis and Nephropathy Syndrome (PDNS). The symptoms of PCVAD develop slowly and progressively, and are often fatal, causing significant economic losses to pig producers worldwide. Vaccines are currently available to pig farms as a prevention measure.
ViPrimePLUS Porcine Circovirus 2 qPCR Kit is intended for research use only.
Kit Component
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PCV2 Primers and Probe Mix (PCV2 PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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RNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Porcine samples (blood, tissues)
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Gene Target
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3’ non coding region (3’NCR).
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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? Internal Extraction Control (IEC) as inhibition control
? Positive control
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Channels
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? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QV5003
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ViPrimePLUS Porcine Circovirus 2 qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Porcine Circovirus 2 qPCR Kit
