Description
The GF-1 Total RNA Extraction Kit is designed for rapid and efficient purification of RNA from various types of samples including cultured animal cells, bacterial cells, animal and plant tissues, and yeast cells. The purification is based on the usage of denaturing agents to provide efficient cell lysis, denaturation of proteins and subsequent release of RNA. Special buffers provided in the kit are optimized to enhance the binding of RNA onto a specially-treated glass filter membrane for efficient recovery of highly pure RNA.
Features
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Yields up to 3μg of total RNA.
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No organic-based extraction required.
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Highly pure RNA ready to use for RT-PCR, Northern blotting, polyA RNA (mRNA) purification, nuclease protection and in vitro translation.
Kit Components
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Buffer TR
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Inhibitor Remover Buffer (concentrate)
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Wash Buffer (concentrate)
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Digestion Buffer
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Digestion Enhancer
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RNase-free Water
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DNase I
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Proteinase K
Ordering Information
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Catalog No
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Description
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Pack Size
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GF-TR-025
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GF-1 Total RNA Extraction Kit
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25 preps
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GF-TR-050
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GF-1 Total RNA Extraction Kit
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50 preps
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GF-TR-100
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GF-1 Total RNA Extraction Kit
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100 preps
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Download
Manual
GF-1 Total RNA Extraction Kit
Publication
This Product Has Been Used In:
El-Taweel, H.M.A., Salah, N.A., Selem, A.K., El-Refaeey, A.A., Abdel-Aziz, A.F. (2017). Visfatin gene expression and oxidative stress in pregnancy induced hypertension, Egyptian Journal of Basic and Applied Sciences (2017).
Etti, I. C., Abdullah, R., Kadir, A., Hashim, N. M., Yeapp, S. K., Imam, M. U., Ramli, F., Malami, I., Lam, K.L., Etti, U., Waziri, P., Rahman, M. (2017). The molecular mechanism of the anticancer effect of Artonin E in MDA-MB 231 triple negative breast cancer cells, . Public Library of Science OneVol. 12, No. 8 (2017).
Liu, T., Liu, F., Wang, C., Wang, Z., Li,Y. (2017) The boosted biomass and lipid accumulation in Chlorella vulgaris by supplementation of synthetic phytohormone analogs. Bioresource Technology. 232. Pp..44-52
Namsen, R., Rojanasthien, N., Sireeratawong, S., Rojsanga, P., Nimlamool, W., Potikanond, S. (2017). Thunbergia laurifolia Exhibits Antifibrotic Effects in Human Hepatic Stellate Cells, Evidence-Based Complementary and Alternative Medicine, Vol. 2017, 9.
Waziri, P.M., Abdullah, R., Yeap, S.W., Omar, A.R., Abdul, A.B., Kassim, N.K., Malami, I., Karunakaran,T., Imam, M.U. 2016. Clausenidin from Clausena excavata induces apoptosis in hepG2 cells via the mitochondrial pathway. Journal of Ethnopharmacology. 194 pp.549-558.
Abu-Elsaad,N.M., Serrya, M.S., El-Karef, A.M., Ibrahim, T.M. (2016).
Kaplan, S. et al. (2016) The Potential of Microarray Databases to Identify Tissue Specific Genes Kafkas üniversitesi Veteriner Fakültesi Dergisi,22(1), p. 29-35.
Loosse, C., Pawlas, M., Bukhari, H.S.S., Maghnouj, A., Hahn, S., Marcus, K., Müller, T. (2016) )Nuclear spheres modulate the expression of BEST1 and GADD45G. Cellular Signalling. 28. Pp.100-109.
Ng, S.L., Harikkrishna, J.A., Bakar,F.A., Yeo, C.C., Cha, T.S. (2016) Heterologous expression of the Streptococcus pneumoniae yoeB and pezT toxin genes is lethal in Chlorella vulgaris. Algal Research. 19 pp.21-29
Saregah, N., et al (2016) Effects of Phenolic-rich Extracts if Clinacanthus nutans on High Fat and High Cholesterol Diet-induced Insulin Resistance BMC Complementary and Alternative Medicine16(88), p.1-11.
Abadi, Z.S.N., Fatemi, F., Khosravani, M., Rahmati, M., Sadroddiny, E (2015). Molecular Cloning of Bone Morphogenetic Protein-2 (BMP- 2) in pGEM-b1 Vector and Transformation into E.coli Rosetta Strain, International Journal of Health Sciences, Vol. 1, No. 3 (2015).
Elias, W.Y. (2015). Assessment of the Osteogenic Potential of Morphogenetic Protein-2 and Insulin-like Growth Factor-I on Adipose Tissue-Derived Stem Cells, Journal of Biomedical Sciences (2015).
Jusoh, M., Loh, S.H., Chuah, T.S., Aziz, A., Cha, T.S. (2015) Elucidating the role of jasmonic acid in oil accumulation, fatty acid composition and gene expression in Chlorella vulgaris (Trebouxiophyceae) during early stationary growth phase. Algal Research. 9. Pp.14-20.
Jusoh, M., Loh, S.H., Chuah, T.S., Aziz, A., Cha. T.S. (2015) Indole-3-acetic acid (IAA) induced changes in oil content, fatty acid profiles and expression of four fatty acid biosynthetic genes in Chlorella vulgaris at early stationary growth phase. Phytochemistry. 111. Pp.65-71.
Moghadam, F.H., et al. (2015) Differentiation of Rat Bone Marrow Mesenchymal Stem Cells into Adipocytes and Cardiomyocytes after Treatment with Platelet Lysate. International Journal of Hematology-Oncology and Stem Cell Research. 10(1), p. 21-29.
Shalabi,M.,Khilo, Kh., Zakaria, M.M., Elsebaei, M.G., Abdo, W., Awadin, W. (2015) Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells. Asian Pacific Journal of Tropical Biomedicine. 5(5) pp.375-381.
Hassanin, A., et al. (2014) Heparin Modulation on Hepatic Nitric Oxide Synthase in Experimental Steatohepatitis. Experimental and Theapeutic Medicine. 8, p. 1551-1558.
Imam, M.U., Ismail, M., Omar, A., Ithnin, H. (2013). The Hypocholesterolemic Effect of Germinated Brown Rice Involves the Upregulation of the Apolipoprotein A1 and Low-Density Lipoprotein Receptor Genes Journal of Diabetes Research (2013).
