Grade
Molecular Biology Grade
Description
DNase I is an endonuclease derived from bovine pancreas that will degrade double-stranded DNA and produce 3’-OH oligonucleotides in the presence of divalent cations. In the reaction solution, with the presence of Mg2+ helps the enzyme to produce nicks in double-stranded DNA, while with the presence of Mn2+, DNase I cleaves both strands of the DNA. DNase I is also useful in nick translation for introducing single-stranded nicks that serve as primer sites for initiation of DNA synthesis and for cloning random DNA fragments by cleaving double-stranded DNA.
DNase I is offered as lyophilized powder with an activity of 500 Kunitz U/mg and is a chromatographically pure preparation. For greatest stability, DNase I is suggested to be dissolved at a concentration of at least 1mg/ml in 50% glycerol with 20mM Tris-HCl, pH7.5, and 1mM MgCl2. This solution can be stored at -20°C for at least a year.
Specification
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Appearance
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White to off-white crystalline powder; a partially purified, lyophilized powder
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Source
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Bovine pancreas All products from animal sources are produced from starting material of USDA-approved origin, collected in United States Department of Agriculture (USDA) or equivalent approved facilities, inspected to be free of disease and suitable for exportation
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Unit Definition
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One unit of DNase I is defined as the amount required to completely degrade 1μg of lambda DNA in 10 minutes at 37°C in 50μl of a buffer containing 40mM Tris-HCl (pH8.0), 2.5mM (up to 10mM) MgSO4, 1mM (up to 10mM) CaCl2. Under these assay conditions one unit of DNase activity is approximately equal to one Kunitz unit.
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Storage
Store at -20°C
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Assay
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Resuls
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Activity
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>500Kunitz U/mg
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Loss on Drying
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≤5.0%
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Residue on Ignition
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≤1.0%
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Ordering Information
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Catalog No
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Description
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Pack Size
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PC0704-25mg
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Deoxyribonuclease I (DNase I)
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25mg
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PC0704-50mg
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Deoxyribonuclease I (DNase I)
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50mg
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PC0704-100mg
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Deoxyribonuclease I (DNase I)
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100mg
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PC0704-1g
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Deoxyribonuclease I (DNase I)
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1g
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Download
MSDS
Deoxyribonuclease I (DNase I)
