Description
Avian Myeloblastosis Virus (AMV) Reverse Transcriptase is an RNA-dependent DNA polymerase (a? holoenzyme) with molecular weight of 157 kDa. It synthesizes a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template.
Concentration
20u/μl
Assay Condition
50mM Tris- HCl (pH8.3), 6mM MgCl2, 40mM KCl, 4mM DTT, 0.5mM [ 3H]-TTP (10-20c/m/pmol), 0.4mM Poly(rA)?(dT)12-18, in a reaction volume of 25μl.
Supplied With
10X Buffer AMV-RT
250mM Tris-HCl (pH 8.3), 500mM KCl, 50mM MgCl2 and 20mM DTT. Store at -70?C.
Storage Buffer
200mM Potassium Phosphate (pH 7.2), 0.2% TritonTM X-100, 2mM DTT and 50% glycerol. Store at -70?C for long term. Store at -20?C for short term (1 month). .
Thermal Inactivation
80?C for 10 minutes.
Unit Definition
1u is defined as the amount of enzyme that is required to incorporate 1nmol of dMTP into an acid-insoluble material in 10 minutes at 37?C using Poly(rA)?(dT)12-18 as a template primer.
Features
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First strand synthesis of cDNA.
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Synthesis of cDNA for cloning.
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cDNA labelling.
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Primer extensions and RNA sequencing
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RT-PCR
-
Dideoxy sequencing of DNA and RNA.
Quality Control
All preparations are assayed for contaminating endonuclease, exonuclease and non-specific RNase activities.
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
ME2301
|
AMV Reverse Transcriptase (Recombinant)
|
500u
|
|
ME2302
|
AMV Reverse Transcriptase (Recombinant)
|
2500u
|
Download
Manual
AMV Reverse Transcriptase (Recombinant)
Publication
This Product Has Been Used In:
Lim et al. (2012) Co-administration of Avian Influenza Virus H5 Plasmid DNA with Chicken IL-15 and IL-18 Enhanced Chickens Immune Responses. BMC Vet Res8: 132.
