Description
The ViPrimePLUS Babesia bigemina qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Babesia bigemina genome in blood samples.
Bovine babesiosis is tick-borne protozoal infection occurring in cattle. The primary responsible species are Babesia bigemina, Babesia bovis and Babesia divergens. Babesia bigemina and Babesia bovis commonly infect cattle, water buffalo and African buffalo, while Babesia divergens favors cattle and reindeer. The transmission is via tick-borne and these are widespread in tropical and subtropical regions. B. bovis (more pathogenic) and B. bigemina are more widely distributed particularly in Africa, Asia, Australia, and Central and South America. B. divergens is economically important in some parts of Europe and possibly northern Africa. The typical clinical manifestation of bovine babesiosis is haemolytic anaemia disease but it varies with species and host immunity. B. bovis infection being more pathogenic with high fatality rate may cause high fever, increased respiratory rate, congestion of conjunctiva, jaundice and neurological signs. B. bigemina infection exhibits signs of fever, parasitemia of 10-30% and red or black urine. Infected animals develop a life-long immunity against re-infection with the same species. Vaccines are commercially available.
ViPrimePLUS Babesia bigemina qPCR Kit is intended for research use only.
Kit Component
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B. bigemina Primers and Probe Mix (B. bigemina PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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DNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Ruminant samples (e.g. blood)
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Gene Target
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18S ribosomal gene
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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? Internal Extraction Control (IEC) as inhibition control
? Positive control
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Channels
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? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QV6003
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ViPrimePLUS Babesia bigemina qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Babesia bigemina qPCR Kit
