Description
The ViPrimePLUS Leishmania major qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Leishmania major genomes in clinical samples (e.g. blood and skin scraping).
Leishmania is a zoonotic protozoan which is transmitted by the bites of female sandfly. There are about 20 species identified as pathogens to human and other mammals, causing leishmaniasis. Different species infections lead to different forms of leishmaniasis. Cutaneous leishmaniasis, which is mainly caused by L. major, L. tropica, L. aethiopica and L. mexicana, is recognized by the presence of large number of skin ulcers. Other species such as L. braziliensis and L. panamensis/L. guyanensis infections may metastasize and affect the mucous membrane of the mouth and throat cavities and surrounding tissues causing mucocutaneous leishmaniasis. Lastly, the visceral leishmaniasis, which is primarily caused by L. donovani complex (L. donovani, L. archibaldi, L. infantum syn. L. chagasi), tends to cause fever, darkened skin swollen lymph nodes and even anemia. Currently, leishmaniasis is currently threatening 350 million people in 88 countries around the world.
ViPrimePLUS Leishmania major qPCR Kit is intended for research use only.
Kit Component
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L. major Primers and Probe Mix (L. major PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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DNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Clinical samples (e.g. blood, skin scraping)
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Gene Target
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NADH dehydrogenase subunit 1 (ND1) kinetoplast encoding gene
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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? Internal Extraction Control (IEC) as inhibition control
? Positive control
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Channels
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? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QM4008
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ViPrimePLUS Leishmania major qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Leishmania major qPCR Kit
