Description
The ViPrimePLUS Ehrlichia qPCR Kit is a Taqman probe-based real-time PCR assay for the detection all species of Ehrlichia genomes in clinical samples (e.g. blood).
Ehrlichia is a genus of small, Gram-negative, essential intracellular bacteria, which belongs to the Anaplasmataceae family. Ehrlichia genomes consist of a circular DNA chromosome of about 1.1 to 1.7Mb varying between species. The genus Ehrlichia comprises of the species E. canis, E. chaffeensis, E. ewingi, E. muris and E. ruminantium. Ticks are used as vectors by Ehrlichiosis for infecting mammals with lone star tick-associated bacteria infecting monocytes and deer-tick associated bacteria infecting granulocytes. The bacteria attach to the host cell via an interaction between the bacterial 120-kD surface-exposed glycoprotein and the host-cell expressed E and L selectins triggering receptor-mediated endocytosis. The bacteria survive in the morula, where they divide by binary fission to produce microcolonies. The infected cell is then destroyed by the morula, releasing bacteria to infect nearby cells. Ehrlichia infects blood cells causing either Human granulocytic ehrlichiosis (HGE) or Human monocytic ehrlichiosis (HME) based on the type of blood cell infected. Symptoms of HME and HE include sudden fever, head and muscle aches and in some cases, a rash. Ehrlichiosis may be severe in immunocompromised hosts, manifesting as a Rocky Mountain spotted fever (RMSF)-like illness that may be fatal. Prevention is mainly by avoiding exposure to ticks as there is no vaccine available but Doxycycline is used in treatment after infection.
ViPrimePLUS Ehrlichia qPCR Kit is intended for research use only.
Kit Component
-
Ehrlichia Primers and Probe Mix (Ehrlichia PPM)
-
Positive Control
-
Internal Extraction Control Primers and Probe Mix (IEC PPM)
-
Internal Extraction Control (IEC DNA)
-
Nuclease Free Water
-
Template Preparation Buffer
Product Specification
|
Technology
|
Taqman probe-based real-time PCR assay
|
|
Type of Nucleic Acid Kit
|
DNA
|
|
Kit Storage
|
Shipped in room temperature, store at -20℃ on arrival
|
|
Sample Material
|
Clinical samples (e.g. blood)
|
|
Gene Target
|
16S ribosomal RNA (18S) gene
|
|
Detection Limit
|
10 to <100 copies per reaction
|
|
Sensitivity & Specificity
|
Ct value 28±3 with probability of 95%
|
|
Controls Included
|
? Internal Extraction Control (IEC) as inhibition control
? Positive control
|
|
Channels
|
? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
|
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
QM4004
|
ViPrimePLUS Ehrlichia qPCR Kit
|
150 reactions
|
Download
Manual
ViPrimePLUS Ehrlichia qPCR Kit
