Description
The ViPrimePLUS Burkholderia mallei qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Burkholderia mallei genome in clinical samples (e.g. nasal discharges, lung, spleen, skin tissue).
Burkholderia mallei is a Gram negative bacterium that affects the equine species causing glanders and with occasional infection in goats, dogs and cats. They are commonly found in soil and the disease is endemic in Africa, Asia, Middle East and South America. The route of transmission is via ingestion of feed and water contaminated with the nasal discharge of infected animals. Cutaneous form of infection is also observed, causing farcy. There are various symptoms portrayed by the infected animals, including mucopurulent nasal discharge, lung lesion, nodules in liver and spleen and high fever during acute infection. Inhalation of aerosolised bacteria may lead to septicemia and chronic infection in other tissue organs. Untreated cases resulted in 95% mortality rate, whereas antibiotic treated cases still resulted in 50% fatality rate. Immunity is not developed in recovered equine, thus are still susceptible to future infection.
ViPrimePLUS Burkholderia mallei qPCR Kit is intended for research use only.
Kit Component
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B. mallei Primers and Probe Mix (B. mallei PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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DNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Avian samples (nasal discharges, lung, spleen, skin tissue)
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Gene Target
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virG and virA genes.
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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? Internal Extraction Control (IEC) as inhibition control
? Positive control
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Channels
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? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QV1011
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ViPrimePLUS Burkholderia mallei qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Burkholderia mallei qPCR Kit
