Description
The ViPrimePLUS Budgerigar Fledgling Disease Virus qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Budgerigar Fledgling Disease Virus genome in blood, faeces, cloacal swabs and fresh or paraffin-embedded tissue.
Budgerigar Fledgling Disease virus is a double stranded DNA avian polyomavirus (APV) which is also known as Papovavirus. It infects the various bird species, especially the fledgling budgerigars and causes the highly contagious Budgerigar Fledgling Disease with a mortality rate ranging from 25-100%. In an outbreak, 100% of birds in the aviary will become infected, but only the young will show symptoms. It is often that young birds died between 10 and 14 days of age (range, 10-28 days), and those that survived were stunted with abnormal feather development. Adults may be asymptomatic, thus becoming a silent carrier. Infected nestling can shed virus for up to 16 weeks, whereas ***** birds may shed virus for only 6 weeks or less. Therefore, real time PCR provides a sensitive, specific and rapid detection of the disease.
ViPrimePLUS Budgerigar Fledgling Disease Virus qPCR Kit is intended for research use only.
Kit Component
-
APV Primers and Probe Mix (APV PPM)
-
Positive Control
-
Internal Extraction Control Primers and Probe Mix (IEC PPM)
-
Internal Extraction Control (IEC DNA)
-
Nuclease Free Water
-
Template Preparation Buffer
Product Specification
|
Technology
|
Taqman probe-based real-time PCR assay
|
|
Type of Nucleic Acid Kit
|
DNA
|
|
Kit Storage
|
Shipped in room temperature, store at -20℃ on arrival
|
|
Sample Material
|
Avian samples (blood, feaeces, cloacal swabs, tissues)
|
|
Gene Target
|
Large T antigen
|
|
Detection Limit
|
10 to <100 copies per reaction
|
|
Sensitivity & Specificity
|
Ct value 28±3 with probability of 95%
|
|
Controls Included
|
? Internal Extraction Control (IEC) as inhibition control
? Positive control
|
|
Channels
|
? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
|
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
QV1010
|
ViPrimePLUS Budgerigar Fledgling Disease Virus qPCR Kit
|
150 reactions
|
Download
Manual
ViPrimePLUS Budgerigar Fledgling Disease Virus qPCR Kit
