Concentration
20u/μl
5 ' . . . C↓T C G A G . . . 3 '
3 ' . . . G A G C T↑C . . . 5 '
Storage Buffer
10mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 100μg/ml BSA and 50% glycerol.
Unit Definition
20u(1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 mins at 50?C in 20μl or 50μl of assay buffer.
Thermal Inactivation
65?C for 20 minutes.
Quality Control Assays
Ligation/ Recutting Assay
After 20-fold overdigestion with Sfr274 I, 90% of the DNA fragments can be ligated and recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 40u of Sfr274 I for 16 hours at 50°C.
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
FDRE1332
|
Sfr274 I
|
25 preps
|
Downloads
Manual
Sfr274 I
