Concentration
10u/μl
5 ' . . . G T↓C G A C . . . 3 '
3 ' . . . C A G C↑T G . . . 5 '
Storage Buffer
10mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 100μg/ml BSA and 50% glycerol.
Unit Definition
10u(1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 mins at 37?C in 20μl or 50μl of assay buffer.
Thermal Inactivation
65?C for 20 minutes
Quality Control Assays
Ligation/ Recutting Assay
After 10-fold overdigestion with Sal I, 90% of the DNA fragments can be ligated and recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 20u of Sal I for 16 hours at 37?C.
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
FDRV1326
|
Sal I
|
100 preps
|
Downloads
Manual
Sal I
