Description
Viva 2-step RT-PCR kit is specially designed to provide reliable synthesis of full-length cDNA and convenient application of cDNA in PCR. M-MuLV Reverse Transcriptase synthesize complementary DNA strand initiating from a specific primer, oligo d(T) or random hexamer. The absence of RNase H enhances the synthesis of long cDNA as RNA strand does not degraded in DNA-RNA hybrid during first strand cDNA synthesis. With variety of kit options for standard PCR and long PCR, Viva 2-step RT-PCR kit provides flexibility in an easy use format.
Features
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Absence of RNase H activity allows high amount of full length cDNA synthesis with RNA templates up to 10kb.
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Wide selection of primers, oligo d(T) or random hexamer. Taq DNA Polymerase and MaxTaq DNA Polymerase for amplification for short and long DNA fragments.
Kit Component
Viva cDNA Synthesis Kit
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10X M-MuLV RT Buffer, 250μl
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10,000u M-MuLV Reverse Transcriptase
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10mM dNTPs mix, 0.25ml
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RNase-free water 3ml
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40μM Oligo d( T ), 100μl
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50ng/μl Random hexamer, 100μl
Viva PCR Kit
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500u Taq DNA Polymerase / 500u Chromo Taq DNA Polymerase / 500u MaxTaq DNA Polymerase / 500u Chromo MaxTaq DNA Polymerase
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10X PCR Reaction Buffers
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50mM MgCl2
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10mM dNTPs Mix, 0.25ml
Storage
Ordering Information
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Catalog No
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Description
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Pack Size
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RTPL12
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Viva 2-Step RT-PCR kit with M-MuLV RT / Taq DNA Polymerase
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100 applications
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RTPL16
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Viva 2-Step RT-PCR Kit with M-MuLV RT / Chromo Taq DNA Polymerase
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100 applications
|
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RTPL22
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Viva 2-Step RT-PCR Kit with M-MuLV RT / MaxTaq DNA Polymerase
|
100 applications
|
|
RTPL26
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Viva 2-Step RT-PCR Kit with M-MuLV RT / Chromo MaxTaq DNA Polymerase
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100 applications
|
Download
Manual
Viva 2-Step RT-PCR kit with M-MuLV RT / Taq DNA Polymerase
Viva 2-Step RT-PCR Kit with M-MuLV RT / Chromo Taq DNA Polymerase
Viva 2-Step RT-PCR Kit with M-MuLV RT / MaxTaq DNA Polymerase
Viva 2-Step RT-PCR Kit with M-MuLV RT / Chromo MaxTaq DNA Polymerase
