Concentration
40u/μl
5 ' . . . G T C T T C ( N ) 1↓. . . 3 '
3 ' . . . C A G A G ( N ) 5↑. . . 5 '
Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 100μg/ml BSA and 50% glycerol.
Unit Definition
40u(1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 minutes at 55?C in 20μl or 50μl of assay buffer.
Thermal Inactivation
65?C for 20 minutes.
Quality Control Assays
Ligation/ Recutting Assay
After 40-fold overdigestion with BstMA I, 90% of the DNA fragments can be ligated and recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 80u of BstMA I for 16 hours at 55?C.
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
FDRE1226
|
BstMA I
|
25 preps
|
Downloads
Manual
BstMA I
