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背景
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Factor H is a fluid phase complement regulatory protein consisting of a single peptide chain of 20 short consensus repeat segments or CCP’s with a molecular weight of approximately 155 KD. 1 Factor H regulates the alternative pathway of the complement system by modifying activity of the “feedback loop.” It does this in three ways. First, it is a co-factor for the serine protease Factor I, which cleaves C3b to iC3b. iC3b has no hemolytic or amplification function, but may be bound by complement receptors. Second, Factor H prevents the formation of and accelerates the disassociation of the alternative pathway C3 convertase, C3bBb from cell surfaces. Finally, Factor H binds to polyanions on host cell surfaces and tissue matrices, such as basement membranes, blocking deposition of C3b. This later activity is leveraged by many pathogens as a mode of complement evasion. 2
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應用
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Applications of this antibody have been evaluated by various research facilities, and include EIA, 5 Western Blot, 4 and FACS. Studies have shown that this antibody functionally inhibits Factor H and can be used to deplete Factor H from Human Serum. 6,7
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參考資料
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1、Pangburn, M.K. Differences between the binding sites of the complement regulatory proteins DAF, CR1 and Factor H on C3 Convertases, J Immunol 136:6, 1986.
2、Kraiczy P, Würrzner, R. Complement escape of human pathogenic bacteria by acquisition of complement regulators, Mol Immunol 43:31-44, 2006.
3、Atkinson, J.P., et al. Complement factor H and the hemolytic uremic syndrome, JEM 204:6, 1245-1248, 2007
4、Sivaprasad, S. and Chong, N.V. The Complement system and age related macular degeneration, Eye 1-6, 2006.
5、Jokiranta, T.S., et al. Analysis of the recognition mechanism of the alternative pathway of complement by monoclonal anti-factor H antibodies: evidence for multiple interactions between H and surface bound C3b, FEBS Ltr 393(2-3):297-302, 1996.
6、Junnikkala, S. et al. Exceptional resistance of Human H2 Glioblastoma Cells to Complement Mediated Killing by Expression and Utilization of Factor H and Factor H Like Protein I, J Immunol 164, 2000.
7、Cheng Z., et al. Complement Factor H as a Marker for Detection of Bladder Cancer, Clin Chem 51:5, 2005.
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