產(chǎn)品介紹
Polyclonal Antiserum to Human C2 Protein
A303
1.0 mL
Ig fraction
≤ 0.1% Sodium Azide
N/A
2°C to 8°C* (≤ 30 days）
For long-term storage (> 30 days), aliquot and store at ≤ –20°C. Avoid repeated freeze-thaw.
For Research Use Only. Not for use in diagnostic procedures
Highly purified human C2s was isolated from normal serum and used to immunize goats. The anti-human C2s polyclonal antisera was tested against normal human serum by double immunodiffusion, quantitative radial immunodiffusion, and ELISA. The antiserum was determined to be monospecific for C2 at varying concentrations.
C2 is a polypeptide, which is present in normal human serum/plasma at approximately 20 μg/mL. This complement protein is synthesized in a variety of locations throughout the body with the main site of synthesis being the liver. It has an approximate molecular weight of 100 kD, however, the molecular weight can vary due to the natural glycosylation of the protein at 8 different sites.
Applications of the C2 polyclonal antisera have been evaluated by various research facilities, and include Western Blot, 1,2,3 Flow Cytometry, 4 and Immunoblot. 5
1、Chang, N., et al. “Regulation of Complement Functional Efficiency by Histidine-Rich Glycoprotein.” Blood (1992): 2973-2980.
2、Harboe, M., et al. “The Down-Stream Effects of Mannan-Induced Lectin Complement Pathway Activation Depend Quantitatively on Alternative Pathway Amplification.” Molecular Immunology (2009): 373-380.
3、Yasojima, K., et al. “Up-Regulated Production and Activation of the Complement System in Alzheimer’s Disease Brain.” American Journal of Pathology (1999): 927-936
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