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產(chǎn)品介紹
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品名
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Polyclonal Antiserum to Human C4 Protein
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貨號
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A305
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規(guī)格
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2.0 mL
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形式
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Whole Antiserum
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防腐劑
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≤ 0.1% Sodium Azide
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物種交叉反應(yīng)
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Baboon, Dog, Horse, Hamster, Rabbit, Rat, Mouse, Cat, Pig
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儲存
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2°C to 8°C (≤ 30 days)
For long-term storage (> 30 days), aliquot and store at ≤ –20°C. Avoid repeated freeze-thaw.
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使用說明
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For Research Use Only. Not for use in diagnostic procedures
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產(chǎn)品描述
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Highly purified human C4 was isolated from normal serum and used to immunize goats. The anti-human C4 polyclonal antisera was tested against normal human plasma by double immunodiffusion, one-dimensional immunoelectrophoresis, quantitative radial immunodifussion, and quantitative rocket immunoelectrophoresis. The antiserum was determined to be monospecific for C4 at varying concentrations.
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背景
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C4 is a plasma protein that is key to the classical and lectin pathways of complement activation. This polypeptide is approximately a 200 kD andis primarily synthesized in single chains by liver hepatocytes. However, synthesis by epithelial cells of the genitourinary and intestinal tract and by phagocytic mononuclear cells has also been reported, and C4 generally circulates as a three-chain molecule instead of a single chain.
The C4 protein contains 3 disulfide bonded subunits: α – 93 kD, β – 75 kD, and γ – 32 kD. The normal concentration of C4 in serum/plasma is approximately 400 μg/mL.
Upon activation of the classical or lectin complement pathway, C4 is cleaved into C4a and C4b fragments. C4a is one of three distinct anaphylatoxins produced directly as a result of complement activation.
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應(yīng)用
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Applications of the C4 polyclonal antisera have been evaluated by various research facilities, and include, Western Blot, 1 IHC, 2 Immunofluorescence, 3 and ELISA. 4
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參考文獻
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1、Avirutnan, P., et al. “Antagonism of the Complement Component C4 by Flavivirus Nonstructural Protein NS1.” J. of Experimental Medicine (2010): 793-806.
2、Truow, L., et al. “C4b-Binding Protein is Present in Affected Areas of Myocardial Infarction During the Acute Inflammatory Phase and Covers a Larger Area than C3.” PLOS One (2008).
3、Jansen, J., et al. “Extensive Complement Activation in Hereditary Porcine Membranoproliferative Glomerulonephritis Type II (Porcine Dense Deposit Disease).” Am. J. of Pathology (1993): 1356-1365
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